C terminal labelling
o Amidation,C-termainal
o Aldehydes
o Alcohols
o pNA (p-Nitroanilide)
o AMC
o AFC
o Cysteamide
o Ester
o N-Alkyl Amides
N terminal labelling
o Acetylated
o Palmytolyl
o HYNIC
o Biotinylated
o Bromoacetylated
o DOTA，DTPA conjugated
o Formylated
o Myristoylated
o Succinylated
Fluorescently labeled modified
o C terminal labelling
AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110
o N terminal labelling
Bodipy-FL, Cy3, Cy5, Texas Red, 5-Tamra, 5-lodoacetamido fluorescein
Rhodamine 110 and Rhodamine B Luciferin, EDANS
FAM, FITC, MCA, Rox, Sulforhodamine 101, 5-TAMRA
Cyclization
o N -> C or Head to Tail
o Disulfide (S-S bond formation) .Trisulfide formation
o Cyclic-natural peptides
Methylation
o Side chain methylation Lys(For),Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, Arg(Me)2 asymmetrical,D-Tyr(Me),D-Tyr(Et)
o N terminal methylation N-Me-Arg,N-Me-Asp, N-Me-Glu, N-Me-Leu, N-Me-Nle, N-Me-Nva, N-Me-Phe, N-Me-S N-Me-Ser, N-Me-Trp, N-Me-Thr, N-Me-Val）
Several common special modification
o Various coupling（BSA, KLH conjugated peptides for antibody production ）
o Phosphoserine, Phosphothreonine, Phosphotyrosine
o Sulfated Tyrosine or Serine
o MAPS (Multiple Antigenic Peptide)
o Glycopeptides
o PEGylation
