Protein antigen
| The service content | cycle |
| Peptide coupling（KLH/BSA/OVA，≤5mg） | 2-3 days |
Protein antigen
| The service content | cycle |
| The gene synthesis | 3-4 weeks |
PCR gene amplification （Provide the template，≤1000bp） | 2-3 weeks |
| Carrier to build | 2-3 weeks |
| Small amounts of expression | 1 week |
| A large number of expression and purification | 2-3 weeks |
Polyclonal antibody production
| The service content | cycle |
| The purity and concentration of the protein antigen detection（SDS-PAGE） | 2-3 days |
| Immune serum collected before | 1 day |
| Animal immunity and serum collection（Two New Zealand rabbit） | 10 week |
| Animal immunity and serum collection（Two New Zealand rabbit，A rapid process） | 6-8 weeks |
| Immune serum titer detection（ELISA） | 2-3 days |
| Immune animal screening before（ELISA） | 2-3 days |
| Immune animal screening before（Western Blot） | 1 week |
| Immune animal screening before（Dot Blot） | 1 week |
| Increase the immune rabbit（1 only） | 6-10 weeks |
| Immune rabbit keep（1 only） | 1 week |
| Increased serum collection（10ml，1 only） | 1 week |
| Extend the immunity and serum collected（10ml，1 only） | 1 week |
| Extend the immunity and serum collected（A goat） | 20 week |
| Extend the immunity and serum collected（Two rats） | 10 week |
| Extend the immunity and serum collected（Four mice） | 10 week |
| Extend the immunity and serum collected（Two guinea pigs） | 11 week |
Monoclonal antibody production
| The service content | cycle |
| The purity and concentration of the protein antigen detection（SDS-PAGE） | 2-3 days |
| Immune serum collected before | 1 day |
| Animal immunity and serum collection（Four mice） | 6-10 weeks |
| Immune serum titer detection（ELISA） | 2-3 days |
| Cell fusion and hybridoma preparation | 10-16 weeks |
| Subtype identification | 1 week |
| Ascites production（Three mice） | 3-4 weeks |
| Transfer cells flask culture(1L) | 5-7 weeks |
Immune animal screening before （ELISA） | 2-3 days |
Immune animal screening before （Western Blot） | 1 week |
Immune animal screening before （Dot Blot） | 1 week |
| Cloning maintain training（1 only） | 1 week |
| Cloning of cryopreserved（1 only） | 1 week |
Antibody purification
| The service content | cycle |
| Saturated ammonium sulfate purification antiserum（≤60ml） | 3-5 days |
| Protein A/G affinity purification antiserum（≤60ml） | 3-5 days |
| Antigen affinity column preparation | 2-3 days |
| Antigen affinity purification antiserum（≤60ml） | 3-5 days |
Antibody detection
| The service content | cycle |
| ELISA detection（1-10 samples） | 2-3 days |
| Cell protein extraction（1 as a sample） | 2-3 days |
| Tissue protein extraction（1 as a sample） | 2-3 days |
Determination of protein concentration （1 as a sample） | 2-3 days |
| Antibodies to Western Blot detection（1 as a sample） | 1 week |
| Antibodies are made in Western Blot detection（ 2-8 samples, provide testing sample concentration ） | 1 week |
| Antibodies are made in Western Blot detection（2-8 samples, the customer does not provide samples） | 2-3 weeks |
| On the basis of internal antibody detection, Western Blot detection target antibody（2-8 samples） | 1-2 weeks |
| Dot Blot test（1-10 samples） | 2-3 days |
IHC testing（Provide slices, 1 picture） | 1 week |
Antibodies to tag
| The service content | cycle |
| HRP labeling（≤5mg） | 1-2 weeks |
Biotin labeling（≤5mg） | 1-2 weeks |
