Antibodies to custom service：
1.Antigen synthesis services: design, synthesis and peptide coupling, protein expression and coupling, etc.
2.Antibody production of experimental animals: rabbit, mouse, rat, chicken, guinea pigs, sheep, etc.
3.Polyclonal antibody preparation services: application type guarantee antibody preparation (ELISA and Western Blot, IHC) and modify specific antibody preparation (phosphorylation, methylation and acetylation).
4.Monoclonal antibody preparation services: application type guarantee antibody preparation (ELISA and Western Blot, IHC) and modify specific antibody preparation (phosphorylation, methylation and acetylation).
5.Antibody purification services: Protein A/G/L purification, antigen affinity purification.
6.Antibody testing services: ELISA (enzyme-linked immunosorbent experiment), the Dot Blot (macular imprinting experiments), Western Blot (Western Blot experiments), IHC (immunohistochemical experiment), etc.
7.Antibody labeling service: HRP (horseradish peroxidase) tag, Biotin (Biotin) tag, FITC tag, etc.
Antibody process monitoring of the project:
1.The antigen design: design of antigen antibody production is one of the key. The company combines genomics, proteomics and structural genomics, according to each of hydrophilic amino acids, surface contact and elastic to predict antigenic determinant, design peptide antigen, and through a third party software validation, ensure its specificity. Screening antigen peptide sequence and analysis of the data submitted to the customer, after discussion with the customer to determine the final antigen sequences.
2.Production: antigen peptide purity is more than 85% (generally more than 90%), far higher than ordinary antibody company USES 75% purity, greatly reduce the impurity of immune response and nonspecific antibody production.
3.Experimental animal immunization: experimental animal breeding and the immune operator, have been antibody production company full-time staff on-site training abroad. For the first time after the immune system and strengthen the immune serum, regularly check the immune effect. Ensure the final titer serum: peptide antigen ELISA > 32000, protein antigen ELISA > 64000, far higher than that of general antibody company guarantee ELISA > 20000 standards.
4.Eventually antibody quality inspection: finished antibodies or antiserum, must pass through all the validation requirements of the customer (ELISA and Western Blot, IHC, etc.). All of the antigen and antibody validation data submitted to customer.
